Biochem/physiol Actions

Reversible: no

Target IC₅₀: 75 nM against DNA topoisomerase II

Primary TargetDNA topoisomerase 2

Product does not compete with ATP.

Cell permeable: yes

General description

A cell-permeable polyanionic, polyaromatic compound used as a powerful inhibitor of cellular processes that are dependent on the formation of protein-nucleic acid complexes. Shown to inhibit apoptotic cell death in various cell types induced by a variety of factors. ATA is also a potent inhibitor of DNA topoisomerase II. Inhibits von Willebrand factor binding to platelets and reduces glutamate-induced neuronal injury. ATA has been reported to stimulate the tyrosine phosphorylation of MAP kinases, Shc proteins, phosphatidylinositol 3-kinase, and phospholipase C-γ. Dye content: ~85%.

A cell-permeable polyanionic, polyaromatic compound used as a powerful inhibitor of cellular processes that are dependent on the formation of protein-nucleic acid complexes. Binds to aFGF and reduces its angiogenic activity. Shown to inhibit apoptotic cell death in various cell types induced by a variety of factors. ATA is a potent inhibitor of DNA topoisomerase II (IC₅₀= 75 nM for the yeast enzyme as measured by relaxation assays) that also acts as a potent inhibitor of angiogenesis. Inhibits von Willebrand factor binding to platelets and reduces glutamate-induced neuronal injury. ATA stimulates the tyrosine phosphorylation of MAP kinases, Shc proteins, phosphatidylinositol 3-kinase, and phospholipase Cγ. Inhibits both major calpain isoforms (IC₅₀ = 22 µM and IC₅₀ = 10 µM for µ-calpain and m-calpain, respectively).

Dye content: ~85%.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lozano, R.M., et al. 1997. Eur. J. Biochem.248, 30.Benchokroun, Y., et al. 1995. Biochem. Pharmacol.49, 305.Okada, N., and Koizumi, S. 1995. J. Biol. Chem.270, 16464.Posner, A., et al. 1995. Biochem. Mol. Biol. Int. 36, 291.Catchpoole, D.R., et al. 1994. Anticancer Res. 14, 853.Csernansky, C.A., et al. 1994. J. Neurosci. Res. 38, 101.Gagliardi, A.R., and Collins, D.C. 1994. Anticancer Res. 14, 475.Gonzalez, R.G., et al. 1980. Biochemistry19, 4299.

Packaging

100 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Warning

Toxicity: Irritant (B)